The Small RNA-seq Lib Prep Kit for Illumina V3 (ABclonal, RK20312) prepares small RNA libraries for Illumina sequencing, compatible with total RNA (100–1000 ng) from animals, plants, serum, plasma, or exosomes. Key steps include 3/5 adapter ligation, RT, PCR amplification, bead purification, and PAGE gel extraction, yielding about 150 bp miRNA libraries (adapter dimers at about 130 bp). Requires Small RNA Adapter Module (RK20358/59/60) for truncated adapters, with PCR ensuring library integrity. Rigorously QC-tested for stability and reproducibility.